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Proteasome 20S alpha 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92293

Novus Biologicals, part of Bio-Techne
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NBP1-92293
NBP1-92293-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome 20S alpha 3 Antibody

Western Blot: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Western Blot: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Western Blot: Proteasome 20S alpha 3 Antibody [NBP1-92293] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Immunocytochemistry/Immunofluorescence: Proteasome 20S alpha 3 Antibody [NBP1-92293] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody [NBP1-92293]

Immunohistochemistry-Paraffin: Proteasome 20S alpha 3 Antibody [NBP1-92293] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Applications for Proteasome 20S alpha 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 20S alpha 3

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Proteasome subunit alpha type-3

Alternate Names

HC8, PSC8, PSMA3

Gene Symbol

PSMA3

Additional Proteasome 20S alpha 3 Products

Product Documents for Proteasome 20S alpha 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Proteasome 20S alpha 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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