Proteasome 20S beta 6 Antibody (2A3-5A8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005694-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A3-5A8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 20S beta 6 Antibodies »

Product Specifications

Immunogen

PSMB6 (AAH00835, 1 a.a. ~ 239 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDELTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA

Specificity

PSMB6 - proteasome (prosome, macropain) subunit, beta type, 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Proteasome 20S beta 6 Antibody (2A3-5A8)

Immunohistochemistry-Paraffin: Proteasome 20S beta 6 Antibody (2A3-5A8) [H00005694-M01]

Immunohistochemistry-Paraffin: Proteasome 20S beta 6 Antibody (2A3-5A8) [H00005694-M01]

Immunohistochemistry-Paraffin: Proteasome 20S beta 6 Antibody (2A3-5A8) [H00005694-M01] - Analysis of monoclonal antibody to PSMB6 on formalin-fixed paraffin-embedded human lymph node tissue. Antibody concentration 1 ~ 10 ug/ml.
Sandwich ELISA: Proteasome 20S beta 6 Antibody (2A3-5A8) [H00005694-M01] - Detection limit for recombinant GST tagged PSMB6 is approximately 3ng/ml as a capture antibody.

Applications for Proteasome 20S beta 6 Antibody (2A3-5A8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta 6

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 1i (proteasome beta 9 subunit).

Long Name

Proteasome subunit beta type-6

Alternate Names

EC 3.4.25.1, LMPY, PSMB6

Entrez Gene IDs

5694 (Human)

Gene Symbol

PSMB6

OMIM

600307 (Human)

UniProt

AAH00835 (Human)

Additional Proteasome 20S beta 6 Products

Product Documents for Proteasome 20S beta 6 Antibody (2A3-5A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 20S beta 6 Antibody (2A3-5A8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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