Proteasome 20S beta2 Antibody (PSH0-28)

Western Blot: Proteasome 20S beta2 Antibody (PSH0-28) [NBP3-32864]

Western Blot: Proteasome 20S beta2 Antibody (PSH0-28) [NBP3-32864] - Western blot analysis of Proteasome 20S beta2 on different lysates with Rabbit anti-Proteasome 20S beta2 antibody (NBP3-32864) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: SK-OV-3 cell lysate
Lane 6: HT-29 cell lysate
Lane 7: PC-3M cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (NBP3-32864) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.