Proteasome 26S S5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19956

Novus Biologicals, part of Bio-Techne
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NBP2-19956
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome 26S S5 Antibodies »

Product Summary for Proteasome 26S S5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S5. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Proteasome 26S S5 Antibody

Western Blot: Proteasome 26S S5 Antibody [NBP2-19956]

Western Blot: Proteasome 26S S5 Antibody [NBP2-19956]

Western Blot: Proteasome 26S S5 Antibody [NBP2-19956] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP2-19956]

Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP2-19956]

Immunohistochemistry-Paraffin: Proteasome 26S S5 Antibody [NBP2-19956] - Human breast cancer, using PSMD5 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Proteasome 26S S5 Antibody [NBP2-19956]

Western Blot: Proteasome 26S S5 Antibody [NBP2-19956]

Western Blot: Proteasome 26S S5 Antibody [NBP2-19956] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.

Applications for Proteasome 26S S5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Proteasome 26S S5

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base. [provided by RefSeq]

Alternate Names

KIAA007226S proteasome non-ATPase regulatory subunit 5, proteasome (prosome, macropain) 26S subunit, non-ATPase, 5,26S proteasome subunit S5B, S5BMGC23145,26S protease subunit S5 basic

Gene Symbol

PSMD5

UniProt

Q16401 (Human)

Additional Proteasome 26S S5 Products

Product Documents for Proteasome 26S S5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Proteasome 26S S5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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