Proteasome subunit beta type 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52080

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Proteasome subunit beta type 4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NRFQIATVTE corresponding to internal region according to NP_002787.2.

Epitope

C-NRFQIATVTE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Proteasome subunit beta type 4 Antibody

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - Staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Proteasome subunit beta type 4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 25-26 kDa kDa band observed in HEK293, HeLa, and K562 lysates and in mouse and rat liver lysates (calculated MW of 29.1 kDa band according to NP_002787.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Proteasome subunit beta type 4

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Long Name

Proteasome subunit beta type-4

Alternate Names

HsBPROS26, HsN3, PROS-26, PROS26, Proteasome chain 3, PSMB4

Entrez Gene IDs

5692 (Human); 19172 (Mouse); 58854 (Rat)

Gene Symbol

PSMB4

UniProt

NP_002787.2 (Human)

Additional Proteasome subunit beta type 4 Products

Product Documents for Proteasome subunit beta type 4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome subunit beta type 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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