Proteasome subunit beta type 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89681

Novus Biologicals, part of Bio-Techne
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NBP1-89681
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Proteasome subunit beta type 4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Proteasome subunit beta type 4 Antibody

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-89681] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Immunocytochemistry/ Immunofluorescence: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Immunocytochemistry/Immunofluorescence: Proteasome subunit beta type 4 Antibody [NBP1-89681] - Staining of human cell line U-251 MG shows localization to centrosome & intermediate filaments.
Immunohistochemistry-Paraffin: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Immunohistochemistry-Paraffin: Proteasome subunit beta type 4 Antibody [NBP1-89681]

Immunohistochemistry-Paraffin: Proteasome subunit beta type 4 Antibody [NBP1-89681] - Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.
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Applications for Proteasome subunit beta type 4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome subunit beta type 4

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Long Name

Proteasome subunit beta type-4

Alternate Names

HsBPROS26, HsN3, PROS-26, PROS26, Proteasome chain 3, PSMB4

Gene Symbol

PSMB4

Additional Proteasome subunit beta type 4 Products

Product Documents for Proteasome subunit beta type 4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Proteasome subunit beta type 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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