Protein Disulfide Isomerase/P4HB Antibody (RL77)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1164

Novus Biologicals, part of Bio-Techne
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NB600-1164-0.025ml
NB600-1164
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Hamster, Xenopus

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # RL77

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Protein Disulfide Isomerase/P4HB Antibodies »

Product Specifications

Immunogen

Purified rat Protein Disulfide Isomerase/P4HB protein.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33806982).

Localization

Endoplasmic reticulum

Specificity

NB600-1164 detects Protein Disulfide Isomerase/P4HB from human, mouse, rat, porcine, xenopus liver tissues as well as hamster (CHO) cells.

Marker

Endoplasmic Reticulum Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody (RL77)

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Analysis of Protein Disulfide Isomerase/P4HB using monoclonal antibody shows staining in A549 Cells.
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody (RL77) [NB600-1164] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
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Applications for Protein Disulfide Isomerase/P4HB Antibody (RL77)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:200 - 1:2000
Application Notes
WB usage was reported in scientific literature (PMID: 21292774). WB: Detects an approx. 59 kDa representing Protein Disulfide Isomerase/P4HB from rat liver extract or a slightly higher protein at 61 kDa representing Protein Disulfide Isomerase/P4HB from human liver extract. IHC: Staining of Protein Disulfide Isomerase/P4HB in rat intestine with ab5484 yields a pattern consistent with cytoplasmic staining. IP: ab5484 has been shown to inhibit the activity of Protein Disulfide Isomerase/P4HB in vitro.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody (RL77)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody (RL77)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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