Protein Disulfide Isomerase/P4HB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84051

Novus Biologicals, part of Bio-Techne
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NBP1-84051
NBP1-84051-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Protein Disulfide Isomerase/P4HB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Protein Disulfide Isomerase/P4HB Recombinant Protein corresponding to amino acids: LPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNF

Marker

Fibroblast Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Disulfide Isomerase/P4HB Antibody

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.
Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051]

Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
View 5 more images

Applications for Protein Disulfide Isomerase/P4HB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Disulfide Isomerase/P4HB

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Long Name

Proline 4-Hydroxylase, Beta Polypeptide

Alternate Names

DSI, ERBA2L, GIT, PDI, PDIA1, PO4DB, PO4HB, PROHB, Thbp

Gene Symbol

P4HB

Additional Protein Disulfide Isomerase/P4HB Products

Product Documents for Protein Disulfide Isomerase/P4HB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein Disulfide Isomerase/P4HB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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