Protein Kinase A regulatory subunit I alpha Antibody (1C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15192

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15192
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1C3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein Kinase A regulatory subunit I alpha Antibodies »

Product Summary for Protein Kinase A regulatory subunit I alpha Antibody (1C3)

Immunogen

A synthesized peptide derived from human Protein Kinase A regulatory subunit I alpha. UniProt ID (P10644).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protein Kinase A regulatory subunit I alpha Antibody (1C3)

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192] - Positive WB detected in: U87 whole cell lysate. All lanes: Protein Kinase A regulatory subunit I alpha antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 39 KDa. Observed band size: 43 KDa.
Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192]

Immunocytochemistry/ Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192]

Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192] - Immunofluorescence staining of Hela cells with at Protein Kinase A regulatory subunit I alpha antibody 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Applications for Protein Kinase A regulatory subunit I alpha Antibody (1C3)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Gene Symbol

PRKAR1A

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Product Specific Notices for Protein Kinase A regulatory subunit I alpha Antibody (1C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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