Protein Kinase A regulatory subunit I alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00289

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein Kinase A regulatory subunit I alpha Antibodies »

Product Summary for Protein Kinase A regulatory subunit I alpha Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human Protein Kinase A regulatory subunit I alpha (Accession#: P10644; Met1-Val381)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Protein Kinase A regulatory subunit I alpha Antibody

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa (We are unsure as to the identity of these extra bands).
Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: Protein Kinase A regulatory subunit I alpha knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa
Immunoprecipitation: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Immunoprecipitation: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289]

Immunoprecipitation: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Protein Kinase A regulatory subunit I alpha was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa

Applications for Protein Kinase A regulatory subunit I alpha Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protein Kinase A regulatory subunit I alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

Alternate Names

PRKAR1, PRKAR1A, TSE1

Gene Symbol

PRKAR1A

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Product Specific Notices for Protein Kinase A regulatory subunit I alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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