Protein Kinase D2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99858

Novus Biologicals, part of Bio-Techne
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NBP2-99858-100ul
NBP2-99858-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRKD2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Protein Kinase D2 (Accession#: NP_057541.2; Met1-Leu878)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Protein Kinase D2 Antibody

Western Blot: Protein Kinase D2 Antibody [NBP2-99858]

Western Blot: Protein Kinase D2 Antibody [NBP2-99858]

Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa
Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858]

Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858]

Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858] - Immunofluorescence staining of Protein Kinase D2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Protein Kinase D2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858]

Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858]

Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858] - Protein Kinase D2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate Lane C: 0.5 mg MCF-7 Whole Cell Lysate 4 ul anti-Protein Kinase D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase D2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa

Applications for Protein Kinase D2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKD2

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Long Name

Protein Kinase D2

Alternate Names

nPKC-D2

Gene Symbol

PRKD2

Additional PRKD2 Products

Product Documents for Protein Kinase D2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein Kinase D2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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