Protein phosphatase 1F Antibody (2A9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009647-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Protein phosphatase 1F Antibodies »

Product Specifications

Immunogen

PPM1F (NP_055449, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSGAPQKSSPMASGAEETPGFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAAALAHEAVSQLLQTDLSEFRKLPR

Reactivity Notes

Human. Other species not tested.

Specificity

PPM1F - protein phosphatase 1F (PP2C domain containing)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Protein phosphatase 1F Antibody (2A9)

Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01]

Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01]

Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01] - PPM1F monoclonal antibody (M01), clone 2A9 Analysis of PPM1F expression in Jurkat.
Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01]

Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01]

Western Blot: Protein phosphatase 1F Antibody (2A9) [H00009647-M01] - Analysis of PPM1F expression in transfected 293T cell line by PPM1F monoclonal antibody (M01), clone 2A9.Lane 1: PPM1F transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Protein phosphatase 1F Antibody (2A9) [H00009647-M01] - Detection limit for recombinant GST tagged PPM1F is approximately 0.3ng/ml as a capture antibody.

Applications for Protein phosphatase 1F Antibody (2A9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protein phosphatase 1F

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Alternate Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaMKPaseCaM-kinase phosphatase, EC 3.1.3.16, FEM-2, hFEM-2, KIAA0015POPX2CAMKP, Partner of PIX 2, PP2C phosphatase, Protein fem-2 homolog, protein phosphatase 1F, protein phosphatase 1F (PP2C domain containing), protein phosphatase, Mg2+/Mn2+ dependent, 1F

Entrez Gene IDs

9647 (Human)

Gene Symbol

PPM1F

UniProt

NP_055449 (Human)

Additional Protein phosphatase 1F Products

Product Documents for Protein phosphatase 1F Antibody (2A9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protein phosphatase 1F Antibody (2A9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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