Protocadherin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47321
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Protocadherin-1 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: AKSGYQAGKKETKDLYAPKPSGKASKGNKSKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Protocadherin-1 Antibody
![Western Blot: Protocadherin-1 Antibody [NBP2-47321]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-Western-Blot-NBP2-47321-img0004.jpg "Western Blot: Protocadherin-1 Antibody [NBP2-47321]")
Western Blot: Protocadherin-1 Antibody [NBP2-47321]
Western Blot: Protocadherin-1 Antibody [NBP2-47321] - Analysis in control (vector only transfected HEK293T lysate) and PCDH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47321-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321]")
Immunocytochemistry/ Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321]
Immunocytochemistry/Immunofluorescence: Protocadherin-1 Antibody [NBP2-47321] - Staining of human cell line HaCaT shows localization to nucleoli, cell junctions & vesicles. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321]](https://resources.bio-techne.com/images/products/Protocadherin-1-Antibody-Immunohistochemistry-NBP2-47321-img0001.jpg "Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321]")
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321]
Immunohistochemistry-Paraffin: Protocadherin-1 Antibody [NBP2-47321] - Staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.Applications for Protocadherin-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Protocadherin-1
Alternate Names
PC42, PCDH1, PCDH42, Protocadherin1
Gene Symbol
PCDH1
Additional Protocadherin-1 Products
Product Documents for Protocadherin-1 Antibody
Product Specific Notices for Protocadherin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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