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Protocadherin-18 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77005

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH18. The immunogen is located within amino acids 50 - 100 of PCDH18. Amino Acid Squence: PNPSTVRFRAMQRGNSPLL

Specificity

At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Protocadherin-18 Antibody - BSA Free

Western Blot: Protocadherin-18 AntibodyBSA Free [NBP1-77005]

Western Blot: Protocadherin-18 AntibodyBSA Free [NBP1-77005]

Western Blot: Protocadherin-18 Antibody [NBP1-77005] - Analysis in HepG2 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: Protocadherin-18 Antibody - BSA Free [NBP1-77005]

Immunocytochemistry/ Immunofluorescence: Protocadherin-18 Antibody - BSA Free [NBP1-77005]

Immunocytochemistry/Immunofluorescence: Protocadherin-18 Antibody [NBP1-77005] - Immunofluorescence of PCDH18 in Mouse Brain cells with PCDH18 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Protocadherin-18 Antibody - BSA Free [NBP1-77005]

Immunohistochemistry-Paraffin: Protocadherin-18 Antibody - BSA Free [NBP1-77005]

Immunohistochemistry-Paraffin: Protocadherin-18 Antibody [NBP1-77005] - Mouse brain tissue with PCDH18 antibody at 2.5 ug/ml.

Applications for Protocadherin-18 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Protocadherin-18

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 18 (PCDH18) was initially identified through screening of human and mouse ESTs using conserved cytoplasmic domain motifs. Tissue screening revealed PCDH18 broad expression in tissues such as brain, liver, heart, kidney, lung, and trachea. PCDH18 expression is also temporally and spatially regulated in the mouse embryonic brain and interacts with Disabled-1, an intracellular adapter protein involved in neuronal migration and cell positioning during mammalian brain development. PCDH18 was also found to be expressed in the developing zebrafish neural tube and central nervous system, lending support to the hypothesis that PCDH18 may play a role during brain development. At least three isoforms of PCDH18 are known to exist. This antibody is predicted to not cross-react with PCDH12.

Alternate Names

KIAA1562, PCDH68L1, Protocadherin18

Gene Symbol

PCDH18

UniProt

Additional Protocadherin-18 Products

Product Documents for Protocadherin-18 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Protocadherin-18 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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