Protocadherin gamma A8 Antibody (1C11)

Novus Biologicals, part of Bio-Techne | Catalog # H00009708-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Protocadherin gamma A8 Antibodies »

Product Specifications

Immunogen

PCDHGA8 (NP_114477, 357 a.a. ~ 444 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LENSLPGTVIAFLSVHDQDSGKNGQVVCYTRDNLPFKLEKSIGNYYRLVTRKYLDRENVSIYNITVMASDLGTPPLSTETQIALHVAD

Reactivity Notes

Human. Other species not tested.

Localization

Cell Membrane

Specificity

PCDHGA8 - protocadherin gamma subfamily A, 8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Protocadherin gamma A8 Antibody (1C11)

Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01]

Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01]

Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01] - Analysis of PCDHGA8 expression in transfected 293T cell line by PCDHGA8 monoclonal antibody (M01), clone 1C11.Lane 1: PCDHGA8 transfected lysate(101.48 KDa).Lane 2: Non-transfected lysate.
Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01]

Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01]

Western Blot: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01] - Analysis of PCDHGA8 over-expressed 293 cell line, cotransfected with PCDHGA8 Validated Chimera RNAi ( Cat # H00009708-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDHGA8 monoclonal antibody (M01), clone 1C11 (Cat # H00009708-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Protocadherin gamma A8 Antibody (1C11) [H00009708-M01] - Detection limit for recombinant GST tagged PCDHGA8 is approximately 0.3ng/ml as a capture antibody.

Applications for Protocadherin gamma A8 Antibody (1C11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for ELISA and RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Protocadherin gamma A8

This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.

Alternate Names

KIAA0327PCDH-GAMMA-A8, PCDH-gamma-A8, protocadherin gamma subfamily A, 8, protocadherin gamma-A8

Entrez Gene IDs

9708 (Human)

Gene Symbol

PCDHGA8

OMIM

606295 (Human)

UniProt

NP_114477 (Human)

Additional Protocadherin gamma A8 Products

Product Documents for Protocadherin gamma A8 Antibody (1C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Protocadherin gamma A8 Antibody (1C11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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