Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2B3

Concentration

1 mg/ml

View all Protogenin Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRTG (NP_776175) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

126.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Protogenin Antibody (OTI2B3)

Western Blot: Protogenin Antibody (OTI2B3) [NBP2-02745]

Western Blot: Protogenin Antibody (OTI2B3) [NBP2-02745]

Western Blot: Protogenin Antibody (2B3) [NBP2-02745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protogenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protogenin.
Immunocytochemistry/ Immunofluorescence: Protogenin Antibody (OTI2B3) [NBP2-02745]

Immunocytochemistry/ Immunofluorescence: Protogenin Antibody (OTI2B3) [NBP2-02745]

Immunocytochemistry/Immunofluorescence: Protogenin Antibody (OTI2B3) [NBP2-02745] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRTG.

Applications for Protogenin Antibody (OTI2B3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Protogenin

PRTG may play a role in anteroposterior axis elongation

Alternate Names

IGDCC5, PRTG, RPTG, Shen-Dan

Entrez Gene IDs

283659 (Human)

Gene Symbol

PRTG

Additional Protogenin Products

Product Documents for Protogenin Antibody (OTI2B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Protogenin Antibody (OTI2B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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