Prox1 Antibody (5G10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30045

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Rat

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, Immunohistochemistry Whole-Mount

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G10

Format

BSA Free

Concentration

1.0 mg/ml

View all Prox1 Antibodies »

Product Specifications

Immunogen

The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786]

Epitope

The homeo prospero domain of Prox1

Reactivity Notes

It is expected to work with all vertebrates including mammals, birds and fish. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Prox1 Antibody (5G10) - BSA Free

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Immunohistochemistry: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Immunohistochemistry: Prox1 Antibody (5G10) [NBP1-30045] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.
Flow Cytometry: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Flow Cytometry: Prox1 Antibody (5G10) - BSA Free [NBP1-30045]

Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications for Prox1 Antibody (5G10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 24654984)

Immunohistochemistry-Paraffin

1:100-1:1000
Application Notes
This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Prox1

Prox1 is a prospero related homeobox gene. Prox1 may play a fundamental role in early development of the CNS. It may also regulate gene expression and development of postmitotic undifferentiated young neurons. Prox1 is expressed in CNS, eye, pancreas, liver and heart tissues, and it is one of the most specific and reliable markers for lymphatic endothelial cells. On a subcellular level Prox1 resides in the nucleus.

Long Name

Prospero-related Homeobox 1

Alternate Names

Homeobox prospero-like protein PROX1, prospero homeobox 1, prospero homeobox protein 1, prospero-related homeobox 1, PROX-1

Entrez Gene IDs

5629 (Human); 19130 (Mouse); 305066 (Rat)

Gene Symbol

PROX1

UniProt

Q92786, P48437 (Mouse)

Additional Prox1 Products

Product Documents for Prox1 Antibody (5G10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Prox1 Antibody (5G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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