PRPF31 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51909

Novus Biologicals, part of Bio-Techne
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NBP1-51909
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PRPF31 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LKVKGEKSGLMST corresponding to C-Terminus according to NP_056444.3.

Epitope

C-LKVKGEKSGLMST

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PRPF31 Antibody

Western Blot: PRPF31 Antibody [NBP1-51909]

Western Blot: PRPF31 Antibody [NBP1-51909]

Western Blot: PRPF31 Antibody [NBP1-51909] - Analysis of PRPF31 in cell line A431 lysate using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PRPF31 Antibody [NBP1-51909]

Western Blot: PRPF31 Antibody [NBP1-51909]

Western Blot: PRPF31 Antibody [NBP1-51909] - (0.3ug/ml) staining of cell line A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PRPF31 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 10 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in A431 lysates (calculated MW of 55.4 kDa band according to NP_056444.3.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRPF31

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 (MIM 180692) RNAs and the 15.5K protein (NHP2L1; MIM 601304). The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state (Liu et al., 2007) [PubMed 17412961].[supplied by OMIM]

Alternate Names

DKFZp566J153, hPrp31, NY-BR-99, Pre-mRNA-processing factor 31, Protein 61K, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), PRP31 pre-mRNA processing factor 31 homolog (yeast), PRP31RP11, Serologically defined breast cancer antigen NY-BR-99, U4/U6 small nuclear ribonucleoprotein Prp31, U4/U6 snRNP 61 kDa protein

Entrez Gene IDs

26121 (Human)

Gene Symbol

PRPF31

UniProt

NP_056444.3 (Human)

Additional PRPF31 Products

Product Documents for PRPF31 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PRPF31 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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