Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Orangutan (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PRPF38A Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 262 to 312 of human Pre-mRNA Processing Factor 38A using the numbering given in entry NP_116253.2 (GeneID 84950).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF38A Antibody

Western Blot: PRPF38A Antibody [NBP2-37697]

Western Blot: PRPF38A Antibody [NBP2-37697]

Western Blot: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PRPF38A Antibody [NBP2-37697]

Immunoprecipitation: PRPF38A Antibody [NBP2-37697]

Immunoprecipitation: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for IP at 6 ug per reaction. PRPF38A was also immunoprecipitated by rabbit anti-PRPF38A antibody. For blotting immunoprecipitated PRPF38A, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PRPF38A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF38A

Alternate Names

FLJ14936, MGC3320, pre-mRNA-splicing factor 38A, Prp38, PRP38 pre-mRNA processing factor 38 (yeast) domain containing A, RP5-965L7.1

Gene Symbol

PRPF38A

UniProt

NP_116253.2 (Human)

Additional PRPF38A Products

Product Documents for PRPF38A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF38A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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