PRPF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83218

Novus Biologicals, part of Bio-Techne
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NBP1-83218
NBP1-83218-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRPF4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QATKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKDGLKAGIEAGNINITSGEVFEIEEHISERQAEVLAEFERRKRARQINVSTDDS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF4 Antibody

Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83218]

Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83218]

Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83218] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PRPF4 antibody NBP1-83218 (A) shows similar protein distribution across tissues to independent antibody NBP1-83217 (B).
Western Blot: PRPF4 Antibody [NBP1-83218]

Western Blot: PRPF4 Antibody [NBP1-83218]

Western Blot: PRPF4 Antibody [NBP1-83218] - Analysis using Anti-PRPF4 antibody NBP1-83218 (A) shows similar pattern to independent antibody NBP1-83217 (B).
Immunocytochemistry/ Immunofluorescence: PRPF4 Antibody [NBP1-83218]

Immunocytochemistry/ Immunofluorescence: PRPF4 Antibody [NBP1-83218]

Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [NBP1-83218] - Staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.
View 5 more images

Applications for PRPF4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRPF4

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by OMIM]

Alternate Names

hPrp4, HPRP4P, HPRP4PRP4/STK/WD splicing factor, PRP4 homolog, PRP4 pre-mRNA processing factor 4 homolog (yeast), Prp4p, PRP4WD splicing factor Prp4, U4/U6 small nuclear ribonucleoprotein Prp4, U4/U6 snRNP 60 kDa protein

Gene Symbol

PRPF4

Additional PRPF4 Products

Product Documents for PRPF4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRPF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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