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PRPF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12635

Novus Biologicals, part of Bio-Techne
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NBP3-12635-100ul
NBP3-12635-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PRPF4 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for PRPF4 Antibody

Western Blot: PRPF4 Antibody [NBP3-12635]

Western Blot: PRPF4 Antibody [NBP3-12635]

Western Blot: PRPF4 Antibody [NBP3-12635] - Lane A: Raji Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293 Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa
Immunoprecipitation: PRPF4 Antibody [NBP3-12635]

Immunoprecipitation: PRPF4 Antibody [NBP3-12635]

Immunoprecipitation: PRPF4 Antibody [NBP3-12635] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-PRPF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRPF4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 58 kDaObserved band size :58 kDa

Applications for PRPF4 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRPF4

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by OMIM]

Alternate Names

hPrp4, HPRP4P, HPRP4PRP4/STK/WD splicing factor, PRP4 homolog, PRP4 pre-mRNA processing factor 4 homolog (yeast), Prp4p, PRP4WD splicing factor Prp4, U4/U6 small nuclear ribonucleoprotein Prp4, U4/U6 snRNP 60 kDa protein

Gene Symbol

PRPF4

Additional PRPF4 Products

Product Documents for PRPF4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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