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PRPF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46212

Novus Biologicals, part of Bio-Techne
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NBP1-46212

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human Pre-mRNA Processing Factor 6 using the numbering given in entry NP_036601.2 (GeneID 24148).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF6 Antibody

Western Blot: PRPF6 Antibody [NBP1-46212]

Western Blot: PRPF6 Antibody [NBP1-46212]

Western Blot: PRPF6 Antibody [NBP1-46212] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46213, which recognizes a downstream epitope.
Immunohistochemistry: PRPF6 Antibody [NBP1-46212]

Immunohistochemistry: PRPF6 Antibody [NBP1-46212]

Immunohistochemistry: PRPF6 Antibody [NBP1-46212] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for PRPF6 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:1,000

Immunohistochemistry-Paraffin

1:100 to 1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF6

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Alternate Names

androgen receptor N-terminal domain transactivating protein-1, ANT-1, bB152O15.1, C20orf14, chromosome 20 open reading frame 14, hPrp6, p102 U5 small nuclear ribonucleoprotein particle-binding protein, pre-mRNA-processing factor 6, Prp6, PRP6 homolog, PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae), PRP6 pre-mRNA processing factor 6 homolog (yeast), putative mitochondrial outer membrane protein import receptor, TOM, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein, U5-102K

Gene Symbol

PRPF6

Additional PRPF6 Products

Product Documents for PRPF6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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