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PRPF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92298

Novus Biologicals, part of Bio-Techne
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NBP1-92298
NBP1-92298-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ARNTLMDMRLSQVSDSVSGQTVVDPKGYLTDLNSMIPTHGGDINDIKKARLLLKSVRETNPHHPPAWIASARLEEVTGKLQVARNL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF6 Antibody

Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298]

Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298]

Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298] - Staining in human fallopian tube and pancreas tissues using anti-PRPF6 antibody. Corresponding PRPF6 RNA-seq data are presented for the same tissues.
Western Blot: PRPF6 Antibody [NBP1-92298]

Western Blot: PRPF6 Antibody [NBP1-92298]

Western Blot: PRPF6 Antibody [NBP1-92298] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: PRPF6 Antibody [NBP1-92298]

Immunocytochemistry/ Immunofluorescence: PRPF6 Antibody [NBP1-92298]

Immunocytochemistry/Immunofluorescence: PRPF6 Antibody [NBP1-92298] - Staining of human cell line CACO-2 shows localization to nuclear speckles. Antibody staining is shown in green.

Applications for PRPF6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRPF6

PRPF6 is encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Alternate Names

androgen receptor N-terminal domain transactivating protein-1, ANT-1, bB152O15.1, C20orf14, chromosome 20 open reading frame 14, hPrp6, p102 U5 small nuclear ribonucleoprotein particle-binding protein, pre-mRNA-processing factor 6, Prp6, PRP6 homolog, PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae), PRP6 pre-mRNA processing factor 6 homolog (yeast), putative mitochondrial outer membrane protein import receptor, TOM, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein, U5-102K

Gene Symbol

PRPF6

Additional PRPF6 Products

Product Documents for PRPF6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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