PRPF8 Antibody (4I1Q8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16440

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16440-100ul
NBP3-16440-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4I1Q8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRPF8 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2335 of human PRPF8 (Q6P2Q9). MADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF8 Antibody (4I1Q8)

Western Blot: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Western Blot: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Western Blot: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Western blot analysis of extracts of various cell lines, using PRPF8 Rabbit mAb (NBP3-16440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Immunocytochemistry/Immunofluorescence: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunofluorescence analysis of U-2 OS cells using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440]

Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunohistochemistry of paraffin-embedded mouse spinal cord using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for PRPF8 Antibody (4I1Q8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRPF8

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Alternate Names

apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, pre-mRNA-processing-splicing factor 8, Prp8, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae), PRP8 pre-mRNA processing factor 8 homolog (yeast), PRP8,220 kDa U5 snRNP-specific protein, PRPC8HPRP8, RP13, Splicing factor Prp8, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Gene Symbol

PRPF8

Additional PRPF8 Products

Product Documents for PRPF8 Antibody (4I1Q8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PRPF8 Antibody (4I1Q8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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