Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PRPF8 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1825 and 1875 of human Pre-mRNA-Processing Factor 8 using the numbering given in entry NP_006436.3 (GeneID 10594).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRPF8 Antibody

Western Blot: PRPF8 Antibody [NBP2-22273]

Western Blot: PRPF8 Antibody [NBP2-22273]

Western Blot: PRPF8 Antibody [NBP2-22273] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273]

Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273]

Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22273] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- PRPF8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: PRPF8 Antibody [NBP2-22273]

Immunoprecipitation: PRPF8 Antibody [NBP2-22273]

Immunoprecipitation: PRPF8 Antibody [NBP2-22273] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22273 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22274 For blotting immunoprecipitated PRPF8, NBP2-22273 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PRPF8 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PRPF8

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Alternate Names

apoptosis-regulated protein 1, apoptosis-regulated protein 2, p220, precursor mRNA processing protein, pre-mRNA-processing-splicing factor 8, Prp8, PRP8 homolog, PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae), PRP8 pre-mRNA processing factor 8 homolog (yeast), PRP8,220 kDa U5 snRNP-specific protein, PRPC8HPRP8, RP13, Splicing factor Prp8, U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Gene Symbol

PRPF8

UniProt

NP_006436.3 (Human)

Additional PRPF8 Products

Product Documents for PRPF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRPF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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