PRR11 Antibody

Western Blot: PRR11 Antibody [NBP3-12614]

Western Blot: PRR11 Antibody [NBP3-12614] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:40 kDaObserved band size:40 kDa

Immunocytochemistry/ Immunofluorescence: PRR11 Antibody [NBP3-12614]

Immunocytochemistry/Immunofluorescence: PRR11 Antibody [NBP3-12614] - Staining of PRR11 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PRR11 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: PRR11 Antibody [NBP3-12614]

Immunoprecipitation: PRR11 Antibody [NBP3-12614] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PRR11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRR11 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa