PSCA Antibody (5C2)
Novus Biologicals, part of Bio-Techne | Catalog # H00008000-M03
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Protein Array, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5C2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PSCA (NP_005663, 23 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CYSCKAQVSNEDCLQVENCTQLGEQCWTARIRAVGLLTVISKGCSLNCVDDSQDYYVGKKNITCCDTDLCNAS
Localization
Cell membrane; Lipid-anchor, GPI-anchor.
Specificity
PSCA - prostate stem cell antigen
Marker
Prostate Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PSCA Antibody (5C2)
![Western Blot: PSCA Antibody (5C2) [H00008000-M03]](https://resources.bio-techne.com/images/products/PSCA-Antibody-5C2-Western-Blot-H00008000-M03-img0002.jpg "Western Blot: PSCA Antibody (5C2) [H00008000-M03]")
Western Blot: PSCA Antibody (5C2) [H00008000-M03]
Western Blot: PSCA Antibody (5C2) [H00008000-M03] - Analysis of PSCA expression in transfected 293T cell line by PSCA monoclonal antibody (M03), clone 5C2. Lane 1: PSCA transfected lysatE (12.9 KDa). Lane 2: Non-transfected lysate.![ELISA: PSCA Antibody (5C2) [H00008000-M03]](https://resources.bio-techne.com/images/products/PSCA-Antibody-5C2-ELISA-H00008000-M03-img0003.jpg "ELISA: PSCA Antibody (5C2) [H00008000-M03]")
ELISA: PSCA Antibody (5C2) [H00008000-M03]
ELISA: PSCA Antibody (5C2) [H00008000-M03] - Detection limit for recombinant GST tagged PSCA is 0.1 ng/ml as a capture antibody.Applications for PSCA Antibody (5C2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PSCA
Long Name
Prostate Stem Cell Antigen
Alternate Names
PRO232
Entrez Gene IDs
8000 (Human)
Gene Symbol
PSCA
OMIM
602470 (Human)
UniProt
Additional PSCA Products
Product Documents for PSCA Antibody (5C2)
Product Specific Notices for PSCA Antibody (5C2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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