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PSF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12589

Novus Biologicals, part of Bio-Techne
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NBP3-12589-50ul
NBP3-12589-100ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PSF3 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for PSF3 Antibody

Western Blot: PSF3 Antibody [NBP3-12589]

Western Blot: PSF3 Antibody [NBP3-12589]

Western Blot: PSF3 Antibody [NBP3-12589] - Lane A: 293T Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:28 kDa
Immunocytochemistry/ Immunofluorescence: PSF3 Antibody [NBP3-12589]

Immunocytochemistry/ Immunofluorescence: PSF3 Antibody [NBP3-12589]

Immunocytochemistry/Immunofluorescence: PSF3 Antibody [NBP3-12589] - Staining of GINS3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GINS3 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Applications for PSF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSF3

PSF3, also known as DNA replication complex GINS protein PSF3, has a 138 amino acid long isoform that is 25 kDa and a short 138 amino acid that is approx. 16 kDa, is located in the nucleus and plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. Studies on this protein have shown a relationship with colon cancer and long QT syndrome. This protein has been studied in DNA replication where it interacts with PSMA4, WDHD1, PSMA6 and MCM6.

Alternate Names

DNA replication complex GINS protein PSF3, GINS complex subunit 3, GINS complex subunit 3 (Psf3 homolog), PSF3FLJ13912

Gene Symbol

GINS3

Additional PSF3 Products

Product Documents for PSF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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