PSGL-1/CD162 Antibody (108)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90249

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 108

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PSGL-1/CD162 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human PSGL-1/CD162 (Uniprot#: Q14242-1; Met1-Gly295).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PSGL-1/CD162 Antibody (108)

Western Blot: PSGL-1/CD162 Antibody (108) [NBP2-90249]

Western Blot: PSGL-1/CD162 Antibody (108) [NBP2-90249]

Western Blot: PSGL-1/CD162 Antibody (108) [NBP2-90249] - Anti-PSGL-1/CD162 rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane.
Immunoprecipitation: PSGL-1/CD162 Antibody (108) [NBP2-90249]

Immunoprecipitation: PSGL-1/CD162 Antibody (108) [NBP2-90249]

Immunoprecipitation: PSGL-1/CD162 Antibody (108) [NBP2-90249] - PSGL-1/CD162 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate. 1 uL anti-SELPLG rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:

Applications for PSGL-1/CD162 Antibody (108)

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (108)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSGL-1/CD162 Antibody (108)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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