PSGL-1/CD162 Antibody (HECA-452)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-78039

Novus Biologicals, part of Bio-Techne
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NB100-78039
NB100-78039SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, ELISA, Flow Cytometry, IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Kappa Clone # HECA-452

Concentration

1.0 mg/ml

View all PSGL-1/CD162 Antibodies »

Product Specifications

Immunogen

Human tonsil stroma

Specificity

CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241).

Clonality

Monoclonal

Host

Rat

Isotype

IgM Kappa

Scientific Data Images for PSGL-1/CD162 Antibody (HECA-452)

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Western Blot: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml.
Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Immunohistochemistry-Paraffin: PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Human tonsil tissue stained with PSGL-1/CD162 Antibody (HECA-452) at 5ug/mL, and Leica Refine DAB kit. IHC-P image submitted by a verified customer review.
Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039]

Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) [NB100-78039] - Cutaneous Lymphocyte Antigen (CLA) Antibody (HECA-452) [NB100-78039] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC.
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Applications for PSGL-1/CD162 Antibody (HECA-452)

Application
Recommended Usage

Flow Cytometry

< = 2 ug/10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 1693467)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-78039 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSGL-1/CD162

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (HECA-452)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSGL-1/CD162 Antibody (HECA-452)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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