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PSGL-1/CD162 Antibody (PSGL1/1567)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07820

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
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NBP3-07820-100ug
NBP3-07820-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # PSGL1/1567

Concentration

0.2 mg/ml

Product Summary for PSGL-1/CD162 Antibody (PSGL1/1567)

Immunogen

Recombinant human PSGL-1/CD162 protein (Uniprot: Q14242)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Column Chromatography. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08695)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for PSGL-1/CD162 Antibody (PSGL1/1567)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Column Chromatography

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PSGL-1/CD162

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

UniProt

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (PSGL1/1567)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSGL-1/CD162 Antibody (PSGL1/1567)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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