PSGL-1/CD162 Antibody (PSGL1/8192R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20632

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PSGL1/8192R

Concentration

0.2 mg/ml

View all PSGL-1/CD162 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human PSGL-1/CD162 protein

Localization

Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human tonsil or spleen.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for PSGL-1/CD162 Antibody (PSGL1/8192R)

PSGL-1/CD162 Antibody (PSGL1/8192R)

PSGL-1/CD162 Antibody (PSGL1/8192R)

Formalin-fixed, paraffin-embedded human spleen stained with PSGL-1/CD162 antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
PSGL-1/CD162 Antibody (PSGL1/8192R)

PSGL-1/CD162 Antibody (PSGL1/8192R)

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using PSGL-1/CD162 antibody (PSGL1/8192R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
PSGL-1/CD162 Antibody (PSGL1/8192R)

PSGL-1/CD162 Antibody (PSGL1/8192R)

Formalin-fixed, paraffin-embedded human tonsil stained with PSGL-1/CD162 antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for PSGL-1/CD162 Antibody (PSGL1/8192R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSGL-1/CD162

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kDa, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Long Name

P-Selectin Glycoprotein Ligand 1

Alternate Names

CD162, PSGL1, SELPLG

Gene Symbol

SELPLG

Additional PSGL-1/CD162 Products

Product Documents for PSGL-1/CD162 Antibody (PSGL1/8192R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSGL-1/CD162 Antibody (PSGL1/8192R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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