PSMB10/MECL1 Antibody (3F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005699-M01

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00005699-M01
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSMB10/MECL1 Antibodies »

Product Specifications

Immunogen

PSMB10 (AAH17198, 1 a.a. ~ 273 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE

Specificity

PSMB10 - proteasome (prosome, macropain) subunit, beta type, 10 (3F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PSMB10/MECL1 Antibody (3F8)

Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01]

Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01]

Western Blot: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - PSMB10 monoclonal antibody (M01), clone 3F8 Analysis of PSMB10 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01]

Immunocytochemistry/ Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01]

Immunocytochemistry/Immunofluorescence: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Analysis of monoclonal antibody to PSMB10 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: PSMB10/MECL1 Antibody (3F8) [H00005699-M01] - Detection limit for recombinant GST tagged PSMB10 is approximately 3ng/ml as a capture antibody.

Applications for PSMB10/MECL1 Antibody (3F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PSMB10/MECL1

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 10

Alternate Names

LMP10, MECl-1, MECL1, Proteasome subunit beta-2i

Entrez Gene IDs

5699 (Human)

Gene Symbol

PSMB10

UniProt

AAH17198 (Human)

Additional PSMB10/MECL1 Products

Product Documents for PSMB10/MECL1 Antibody (3F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSMB10/MECL1 Antibody (3F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.