PSMB10/MECL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51958

Novus Biologicals, part of Bio-Techne
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NBP1-51958
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PSMB10/MECL1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PTEPVKRSGRYH corresponding to internal region according to NP_002792.1.

Epitope

C-PTEPVKRSGRYH

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMB10/MECL1 Antibody

Western Blot: PSMB10/MECL1 Antibody [NBP1-51958]

Western Blot: PSMB10/MECL1 Antibody [NBP1-51958]

Western Blot: PSMB10/MECL1 Antibody [NBP1-51958] - Analysis of PSMB10 in Human Lung lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958]

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958]

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human small intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958]

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958]

Immunohistochemistry-Paraffin: PSMB10/MECL1 Antibody [NBP1-51958] - Staining of paraffin embedded human tonsil. Antbody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PSMB10/MECL1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 26 kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9 kDa according to NP_002792.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMB10/MECL1

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 10

Alternate Names

LMP10, MECl-1, MECL1, Proteasome subunit beta-2i

Entrez Gene IDs

5699 (Human); 19171 (Mouse); 291983 (Rat)

Gene Symbol

PSMB10

UniProt

NP_002792.1 (Human)

Additional PSMB10/MECL1 Products

Product Documents for PSMB10/MECL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMB10/MECL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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