PSMB7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52081

Novus Biologicals, part of Bio-Techne
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NBP1-52081
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PSMB7 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RNAVLEADFAKR corresponding to internal region according to NP_002790.1.

Epitope

C-RNAVLEADFAKR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMB7 Antibody

Western Blot: PSMB7 Antibody [NBP1-52081]

Western Blot: PSMB7 Antibody [NBP1-52081]

Western Blot: Proteasome 20S beta 7 Antibody [NBP1-52081] - NBP1-52081 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PSMB7 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of HEK 293, HeLa and Jurkat and of mouse Fetal brain (calculated MW of 30.0 kDa band according to NP_002790.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMB7

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Entrez Gene IDs

5695 (Human); 19177 (Mouse); 85492 (Rat)

Gene Symbol

PSMB7

UniProt

NP_002790.1 (Human)

Additional PSMB7 Products

Product Documents for PSMB7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PSMB7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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