Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PSMC1 Antibodies »

Product Summary for PSMC1 Antibody

Immunogen

This antibody maps to a region between residue 390 and 440 of human Proteasome (prosome, macropain) Subunit, ATPase, 1 using the numbering given in entry NP_002793.2 (GeneID 5700).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMC1 Antibody

Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]

Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]

Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]

Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857]

Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: PSMC1 Antibody [NBP2-14857]

Immunoprecipitation: PSMC1 Antibody [NBP2-14857]

Immunoprecipitation: PSMC1 Antibody [NBP2-14857] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC1 was successfully immunoprecipitated by rabbit anti-PSMC1 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for PSMC1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSMC1

Alternate Names

26S protease regulatory subunit 4, 26S proteasome AAA-ATPase subunit RPT2, MGC24583, P26s4, p56, proteasome (prosome, macropain) 26S subunit, ATPase, 1, proteasome 26S ATPase subunit 1, Proteasome 26S subunit ATPase 1, proteasome 26S subunit, ATPase, 1, S4MGC8541

Gene Symbol

PSMC1

UniProt

NP_002793.2 (Human)

Additional PSMC1 Products

Product Documents for PSMC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSMC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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