PSMD11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46191
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Drosophila
Cited:
Human, Mouse, Insect - Drosophila
Predicted:
Bovine (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 175 and 225 of human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 11 using the numbering given in entry NP_002806.2 (GeneID 5717).
Reactivity Notes
X. tropicalis (100%). Use in Drosophila reported in scientific literature (PMID:31002009).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PSMD11 Antibody
![Western Blot: PSMD11 Antibody [NBP1-46191]](https://resources.bio-techne.com/images/products/PSMD11-Antibody-Western-Blot-NBP1-46191-img0003.jpg "Western Blot: PSMD11 Antibody [NBP1-46191]")
Western Blot: PSMD11 Antibody [NBP1-46191]
Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome subunits Rpn6 (PSMD11), Rpt6, alpha7 and beta5 expression levels after cell exposure to the shown concentrations of 6BIO for 36 h. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-11662-7), licensed under a CC-BY license.![Western Blot: PSMD11 Antibody [NBP1-46191]](https://resources.bio-techne.com/images/products/PSMD11-Antibody-Western-Blot-NBP1-46191-img0002.jpg "Western Blot: PSMD11 Antibody [NBP1-46191]")
Western Blot: PSMD11 Antibody [NBP1-46191]
Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PSMD11 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PSMD11 was also immunoprecipitated by rabbit anti-PSMD11 antibody NBP1-46192, which recognizes a downstream epitope.Applications for PSMD11 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PSMD11
Alternate Names
MGC3844,26S proteasome regulatory subunit RPN6, p44.5,26S proteasome regulatory subunit 9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5, Rpn6,26S proteasome regulatory subunit S9, S9,26S proteasome non-ATPase regulatory subunit 11
Gene Symbol
PSMD11
Additional PSMD11 Products
Product Documents for PSMD11 Antibody
Product Specific Notices for PSMD11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...