PSMD13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80961

Novus Biologicals, part of Bio-Techne
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NBP1-80961
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PSMD13 Antibodies »

Product Summary for PSMD13 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEKTREKVKSSDEAVILCKTAIGALKLNIGDLQVTKETIEDVEEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD13 Antibody

Western Blot: PSMD13 Antibody [NBP1-80961]

Western Blot: PSMD13 Antibody [NBP1-80961]

Western Blot: PSMD13 Antibody [NBP1-80961] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PSMD13 Antibody [NBP1-80961]

Immunocytochemistry/ Immunofluorescence: PSMD13 Antibody [NBP1-80961]

Immunocytochemistry/Immunofluorescence: PSMD13 Antibody [NBP1-80961] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & nuclear speckles.
Immunohistochemistry-Paraffin: PSMD13 Antibody [NBP1-80961]

Immunohistochemistry-Paraffin: PSMD13 Antibody [NBP1-80961]

Immunohistochemistry-Paraffin: PSMD13 Antibody [NBP1-80961] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
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Applications for PSMD13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD13

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq]

Alternate Names

HSPC027,26S proteasome regulatory subunit RPN9, p40.5,26S proteasome subunit p40.5,26S proteasome regulatory subunit p40.5, proteasome (prosome, macropain) 26S subunit, non-ATPase, 13,26S proteasome regulatory subunit S11, Rpn9,26S proteasome non-ATPase regulatory subunit 13, S11

Entrez Gene IDs

5719 (Human)

Gene Symbol

PSMD13

UniProt

Q9UNM6 (Human)

Additional PSMD13 Products

Product Documents for PSMD13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMD13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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