PSMD14 Antibody (5V0H3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16837
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5V0H3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 112-210 of human PSMD14 (O00487). YHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PSMD14 Antibody (5V0H3)
Western Blot: PSMD14 Antibody (5V0H3) [NBP3-16837]
Western Blot: PSMD14 Antibody (5V0H3) [NBP3-16837] - Western blot analysis of extracts of various cell lines, using PSMD14 Rabbit mAb (NBP3-16837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: PSMD14 Antibody (5V0H3) [NBP3-16837]
Immunocytochemistry/Immunofluorescence: PSMD14 Antibody (5V0H3) [NBP3-16837] - Immunofluorescence analysis of U-2 OS cells using PSMD14 Rabbit mAb (NBP3-16837) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PSMD14 Antibody (5V0H3) [NBP3-16837]
Immunohistochemistry-Paraffin: PSMD14 Antibody (5V0H3) [NBP3-16837] - Immunohistochemistry of paraffin-embedded mouse heart using PSMD14 Rabbit mAb (NBP3-16837) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for PSMD14 Antibody (5V0H3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PSMD14
Alternate Names
EC 3.1.2.15, EC 3.4.19.-, pad1, POH126S proteasome non-ATPase regulatory subunit 14,26S proteasome regulatory subunit RPN11, proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, Rpn11, RPN11,26S proteasome-associated PAD1 homolog 1
Gene Symbol
PSMD14
Additional PSMD14 Products
Product Documents for PSMD14 Antibody (5V0H3)
Product Specific Notices for PSMD14 Antibody (5V0H3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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