Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PSME4 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator subunit 4 using the numbering given in entry NP_055429.2 (GeneID 23198).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSME4 Antibody

Western Blot: PSME4 Antibody [NBP2-22236]

Western Blot: PSME4 Antibody [NBP2-22236]

Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236]

Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236]

Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236] - Human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- PSME4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: PSME4 Antibody [NBP2-22236]

Immunoprecipitation: PSME4 Antibody [NBP2-22236]

Immunoprecipitation: PSME4 Antibody [NBP2-22236] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. NBP2-22236 used for IP at 6 ug/mg lysate. PSME4 was also immunoprecipitated by rabbit anti-PSME4 antibody. For blotting immunoprecipitated PSME4, NBP2-22236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for PSME4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSME4

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. PA200 has been identified as a 200 kDa nuclear protein that activates the 26S proteasome complex. Studies have shown that, in human tissues, PA200 mRNA levels are highest in testis, but is also present in liver, spleen, brain, heart, kidney and lung. Evidence suggests that this protein is involved in DNA repair, possibly by recruiting the proteasome to double-strand DNA breaks.

Alternate Names

proteasome (prosome, macropain) activator subunit 4

Gene Symbol

PSME4

UniProt

NP_055429.2 (Human)

Additional PSME4 Products

Product Documents for PSME4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PSME4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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