PSME4 Antibody

Western Blot: PSME4 Antibody [NBP3-32869]

Western Blot: PSME4 Antibody [NBP3-32869] - Western blot analysis of PSME4 on different lysates with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/500 dilution.

Lane 1: 293T cell lysate (10 ug/Lane)
Lane 2: Hela cell lysate (10 ug/Lane)
Lane 3: Jurkat cell lysate (10 ug/Lane)
Lane 4: RAW264.7 cell lysate (10 ug/Lane)
Lane 5: Rat skeletal muscle tissue lysate (20 ug/Lane)

Predicted band size: 211 kDa
Observed band size: 211 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32869) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PSME4 Antibody [NBP3-32869]

Immunohistochemistry: PSME4 Antibody [NBP3-32869] - Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PSME4 antibody (NBP3-32869) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32869) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.