Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PSMF1 Antibodies »

Product Summary for PSMF1 Antibody (OTI2D7) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human PSMF1(NP_848693) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMF1 Antibody (OTI2D7) - Azide and BSA Free

Western Blot: PSMF1 Antibody (OTI2D7)Azide and BSA Free [NBP2-73707]

Western Blot: PSMF1 Antibody (OTI2D7)Azide and BSA Free [NBP2-73707]

Western Blot: PSMF1 Antibody (OTI2D7) - Azide and BSA Free [NBP2-73707] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMF1.
Immunocytochemistry/ Immunofluorescence: PSMF1 Antibody (OTI2D7) - Azide and BSA Free [NBP2-73707]

Immunocytochemistry/ Immunofluorescence: PSMF1 Antibody (OTI2D7) - Azide and BSA Free [NBP2-73707]

Immunocytochemistry/Immunofluorescence: PSMF1 Antibody (OTI2D7) - Azide and BSA Free [NBP2-73707] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PSMF1.
Western Blot: PSMF1 Antibody (OTI2D7)Azide and BSA Free [NBP2-73707]

Western Blot: PSMF1 Antibody (OTI2D7)Azide and BSA Free [NBP2-73707]

Western Blot: PSMF1 Antibody (OTI2D7) - Azide and BSA Free [NBP2-73707] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Applications for PSMF1 Antibody (OTI2D7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMF1

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Alternate Names

hPI31, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), proteasome inhibitor hP131 subunit, proteasome inhibitor PI31 subunit

Gene Symbol

PSMF1

Additional PSMF1 Products

Product Documents for PSMF1 Antibody (OTI2D7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PSMF1 Antibody (OTI2D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.