PSMG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36951

Novus Biologicals, part of Bio-Techne
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NBP1-36951
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PSMG1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLMTTNEIQSNIYT corresponding to C-Terminus according to NP_982257.1.

Epitope

C Terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_003711.1; NP_982257.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMG1 Antibody

Immunocytochemistry/ Immunofluorescence: PSMG1 Antibody [NBP1-36951]

Immunocytochemistry/ Immunofluorescence: PSMG1 Antibody [NBP1-36951]

Immunocytochemistry/Immunofluorescence: PSMG1 Antibody [NBP1-36951] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Golgi apparatus and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Flow Cytometry: PSMG1 Antibody [NBP1-36951]

Flow Cytometry: PSMG1 Antibody [NBP1-36951]

Flow Cytometry: PSMG1 Antibody [NBP1-36951] - analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: PSMG1 Antibody [NBP1-36951]

Immunocytochemistry/ Immunofluorescence: PSMG1 Antibody [NBP1-36951]

Immunocytochemistry/Immunofluorescence: PSMG1 Antibody [NBP1-36951] - analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Applications for PSMG1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and U2OS cells.
Flow Cytometry: Analysis of Jurkat cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMG1

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization

Alternate Names

C21-LRP, C21LRPc21-LRP, Chromosome 21 leucine-rich protein, Down syndrome critical region gene 2, DSCR2leucine rich protein C21-LRP, LRPC21, PAC-1, PAC1Down syndrome critical region protein 2, proteasome (prosome, macropain) assembly chaperone 1, proteasome assembling chaperone 1, proteasome assembly chaperone 1

Entrez Gene IDs

8624 (Human); 56088 (Mouse); 288236 (Rat)

Gene Symbol

PSMG1

UniProt

NP_003711.1 (Human)

Additional PSMG1 Products

Product Documents for PSMG1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PSMG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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