PTBP1 Antibody (9M6A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16197

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16197-100ul
NBP3-16197-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9M6A8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PTBP1 Antibodies »

Product Summary for PTBP1 Antibody (9M6A8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (P26599). MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTBP1 Antibody (9M6A8)

Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197]

Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197]

Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197] - Western blot analysis of extracts of various cell lines, using PTBP1 Rabbit mAb (NBP3-16197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197]

Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197]

Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunofluorescence analysis of U-2 OS cells using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PTBP1 Antibody (9M6A8) [NBP3-16197]

Immunohistochemistry-Paraffin: PTBP1 Antibody (9M6A8) [NBP3-16197]

Immunohistochemistry-Paraffin: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for PTBP1 Antibody (9M6A8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTBP1

PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

Heterogeneous nuclear ribonucleoprotein I, heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, HNRNPI, HNRNP-I, HNRPI, MGC10830, MGC8461, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoproteinI), polypyrimidine tract binding protein 1, polypyrimidine tract-binding protein 1, pPTB, PTB-1, PTB2, PTB3, PTB4, PTB57 kDa RNA-binding protein PPTB-1, PTB-T, RNA-binding protein

Gene Symbol

PTBP1

Additional PTBP1 Products

Product Documents for PTBP1 Antibody (9M6A8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTBP1 Antibody (9M6A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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