PTBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1310

Novus Biologicals, part of Bio-Techne
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NB100-1310
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PTBP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence DGIVPDIAVGTKR-C corresponding to N-Terminus according to NP_114367.1, NP_114368.1, NP_787041.1.

Specificity

This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PTBP1 Antibody

Western Blot: PTBP1 Antibody [NB100-1310]

Western Blot: PTBP1 Antibody [NB100-1310]

Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310]

Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310]

Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310] - (2ug/ml) Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Western Blot: PTBP1 Antibody [NB100-1310]

Western Blot: PTBP1 Antibody [NB100-1310]

Western Blot: PTBP1 Antibody [NB100-1310] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
View 2 more images

Applications for PTBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2-4 ug/ml

Peptide ELISA

detection Limit 1:32000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human liver and ovary lysates, and 60 kDa band observed in nuclear lysates of HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2 kDa band according to NP_114368.1). IHC-P: Human kidney shows nuclear membrane staining in all the convoluted tubules.
IHC: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTBP1

Polypyrimidine tract-binding protein (PTB), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a 59.6-kD nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. The hnRNP I protein shows an unusual pattern of nuclear localization. It has been implicated in pre-mRNA splicing.

Alternate Names

Heterogeneous nuclear ribonucleoprotein I, heterogeneous nuclear ribonucleoprotein polypeptide I, hnRNP I, HNRNPI, HNRNP-I, HNRPI, MGC10830, MGC8461, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoproteinI), polypyrimidine tract binding protein 1, polypyrimidine tract-binding protein 1, pPTB, PTB-1, PTB2, PTB3, PTB4, PTB57 kDa RNA-binding protein PPTB-1, PTB-T, RNA-binding protein

Entrez Gene IDs

5725 (Human); 19205 (Mouse); 29497 (Rat)

Gene Symbol

PTBP1

UniProt

NP_002810 (Human)

Additional PTBP1 Products

Product Documents for PTBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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