PTF1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60953

Novus Biologicals, part of Bio-Techne
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NB100-60953
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PTF1A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-WTDEKQLKEQN corresponding to internal region according to NP_835455.1.

Epitope

WTDEKQLKEQN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PTF1A Antibody

Western Blot: PTF1A Antibody [NB100-60953]

Western Blot: PTF1A Antibody [NB100-60953]

Western Blot: PTF1A Antibody [NB100-60953] - Staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Western Blot: PTF1A Antibody [NB100-60953]

Western Blot: PTF1A Antibody [NB100-60953]

Western Blot: PTF1A Antibody [NB100-60953] - Staining at 1 ug/mL antibody of U2OS (A) + peptide (B), 0.5ug/ml antibody Jurkat (C) + peptide (D) and NIH3T3 (E) + peptide (F) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for PTF1A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/mL
Application Notes
Western blot: Approx 37 kDa observe in Human Pancreas lysates and approx.38-40 kDa in lysates of cell lines Jurkat, U2OS and NIH3T3, calculated MW of 35.0kDa according to Human NP_835455.1 and 35.2 kDa according to Mouse NP_061279.2.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTF1A

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.

Long Name

Pancreas-specific Transcription Factor 1a

Alternate Names

PTF1-p48

Entrez Gene IDs

256297 (Human); 19213 (Mouse); 117034 (Rat)

Gene Symbol

PTF1A

UniProt

Q7RTS3 (Human)

Additional PTF1A Products

Product Documents for PTF1A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PTF1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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