PTH Antibody (JE66-58)

Western Blot: PTH Antibody (JE66-58) [NBP3-32717]

Western Blot: PTH Antibody (JE66-58) [NBP3-32717] - Western blot analysis of PTH on different lysates with Rabbit anti-PTH antibody (NBP3-32717) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (20 ug/Lane)
Lane 2: CAL-62 cell lysate (20 ug/Lane)
Lane 3: A549 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Mouse thymus tissue lysate (40 ug/Lane)
Lane 6: Rat thymus tissue lysate (40 ug/Lane)

Predicted band size: 13 kDa
Observed band size: 16 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32717) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PTH Antibody (JE66-58) [NBP3-32717]

Immunohistochemistry: PTH Antibody (JE66-58) [NBP3-32717] - Immunohistochemical analysis of paraffin-embedded human parathyroid tissue with Rabbit anti-PTH antibody (NBP3-32717) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32717) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.