Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PTIP Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human PAX transcription activation domain interacting protein 1 using the numbering given in Jowsey et akl., 2004, J. Biol. Chem. 279(53):55562-55569 (GeneID 22976).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTIP Antibody

Western Blot: PTIP Antibody [NB100-577]

Western Blot: PTIP Antibody [NB100-577]

Western Blot: PTIP Antibody [NB100-577] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PTIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Immunoprecipitation: PTIP Antibody [NB100-577]

Immunoprecipitation: PTIP Antibody [NB100-577]

Immunoprecipitation: PTIP Antibody [NB100-577] - Detection of human PTIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTIP antibody NB100-577 used for IP at 3 ug per reaction. PTIP was also immunoprecipitated by rabbit anti-PTIP antibody from Company B. For blotting immunoprecipitated PTIP, NB100-577 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PTIP Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PTIP

PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP and in BRCA1 serve as phosphoserine or phosphothreonine specific binding modules that recognize substrates phosphorylated by the kinases ATM and ATR in response to gamma irradiation.

Alternate Names

CAGF28, PACIP1, PAX interacting (with transcription-activation domain) protein 1, PAX transactivation activation domain-interacting protein, PAX transcription activation domain interacting protein 1 like, PAX-interacting protein 1, PAXIP1LCAGF29, protein encoded by CAG trinucleotide repeats, PTIPFLJ41049, TNRC2

Entrez Gene IDs

22976 (Human)

Gene Symbol

PAXIP1

Additional PTIP Products

Product Documents for PTIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PTIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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