PTK7/CCK4 Antibody (4F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005754-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PTK7/CCK4 Antibodies »

Product Specifications

Immunogen

PTK7 (NP_002812, 36 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQ

Specificity

PTK7 - protein tyrosine kinase 7

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PTK7/CCK4 Antibody (4F5)

Western Blot: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

Western Blot: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

Western Blot: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Analysis of PTK7 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Analysis of monoclonal antibody to PTK7 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

ELISA: PTK7/CCK4 Antibody (4F5) [H00005754-M06]

ELISA: PTK7/CCK4 Antibody (4F5) [H00005754-M06] - Detection limit for recombinant GST tagged PTK7 is approximately 1ng/ml as a capture antibody.

Applications for PTK7/CCK4 Antibody (4F5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTK7/CCK4

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene.

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Entrez Gene IDs

5754 (Human); 71461 (Mouse); 301242 (Rat)

Gene Symbol

PTK7

OMIM

601890 (Human)

UniProt

NP_002812

Additional PTK7/CCK4 Products

Product Documents for PTK7/CCK4 Antibody (4F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PTK7/CCK4 Antibody (4F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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