Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PTP epsilon Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

Western Blot: PTP epsilon Antibody (OTI5F2)Azide and BSA Free [NBP2-73713]

Western Blot: PTP epsilon Antibody (OTI5F2)Azide and BSA Free [NBP2-73713]

Western Blot: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTP epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTP epsilo
Immunocytochemistry/ Immunofluorescence: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713]

Immunocytochemistry/ Immunofluorescence: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713]

Immunocytochemistry/Immunofluorescence: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTP epsilon.
Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713]

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713]

Immunohistochemistry: PTP epsilon Antibody (OTI5F2) - Azide and BSA Free [NBP2-73713] - Staining of paraffin-embedded liver tissue using anti-PTP epsilon mouse monoclonal antibody.
View 5 more images

Applications for PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTP epsilon

PTP epsilon, an R4 receptor protein tyrosine phosphatase, is composed of a short extracellular and two cyplasmic protein phosphatase domains and has been shown to affect neuronal differentiation, endothelial cell growth, vascular development, and possibly mammary tumor development. It has been shown that the cytosolic form of PTP epsilon can be induced by IL-1 and TNF alpha in humans and is a negative regulator of IL-6- and LIF-induced Jak-STAT kinase signaling in rats. Four alternatively spliced protein isoforms have been documented for PTP epsilon: a membrane form, a cytosolic form, the p65 form, which results from translation using an internal initiation codon, and the p67 form, which results from a specific proteolytic cleavage of wild-type PTP epsilon. PTP epsilon expression has been documented in human vascular endothelial cells, astrocytoma cells, and in IW32 erythroleukemia overexpressing p53. PTP epsilon expression has been documented in animal brain, breast, ganglion, heart, kidney, liver, lung, nerve, spinal cord, spleen, testis, and vessel. The membrane and cytosolic forms of PTP epsilon show different tissue expression patterns. ESTs have been isolated from numerous human tissue libraries, including normal human blood, brain, testis, and thyroid, and cancerous human blood, brain, breast, colon, embryo, head/neck, pancreas, and skin.

Alternate Names

DKFZp313F1310, EC 3.1.3.48, FLJ57799, FLJ58245, HPTPE, protein tyrosine phosphatase epsilon, protein tyrosine phosphatase, receptor type, E, protein tyrosine phosphatase, receptor type, epsilon polypeptide, Protein-tyrosine phosphatase epsilon, PTPE, receptor-type tyrosine-protein phosphatase epsilon, R-PTP-epsilon

Gene Symbol

PTPRE

Additional PTP epsilon Products

Product Documents for PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PTP epsilon Antibody (OTI5F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.